@@ -173,7 +173,7 @@ Next we'll initialize 3 more variables named `genome`, `transcriptome` and `gtf`
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# directory with the genome and transcriptome index files + name of the gene annotation file
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genome=/n/groups/hbctraining/intro_rnaseq_hpc/reference_data_ensembl38/ensembl38_STAR_index
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- transcriptome=/n/groups/hbctraining/rna-seq_2019_02/reference_data/ salmon_index
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+ transcriptome=/n/groups/hbctraining/rna-seq_2023_02/ salmon_index/ref-transcripts
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gtf=/n/groups/hbctraining/intro_rnaseq_hpc/reference_data_ensembl38/Homo_sapiens.GRCh38.92.1.gtf
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```
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@@ -295,7 +295,7 @@ The <code>rnaseq_analysis_on_input_file.sh</code> should look like:
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<pre >
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# ; !/bin/bash/
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# ; This script takes a FASTQ file of RNA-seq data, runs FastQC, STAR, Qualimap and Salmon.
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- # ; USAGE: sh rnaseq_analysis_on_input_file.sh < name of FASTQ file >
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+ # ; USAGE: sh rnaseq_analysis_on_input_file.sh & lt ; name of FASTQ file& gt ;
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# ; change directories to /n/scratch/ so that all the analysis is stored there.<br >
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cd /n/scratch/users/r/$USER/rnaseq_hbc-workshop/<br >
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# ; initialize a variable with an intuitive name to store the name of the input FASTQ file<br >
@@ -307,7 +307,7 @@ echo "Sample name is ${samplename}"<br>
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cores=6<br >
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# ; directory with the genome and transcriptome index files + name of the gene annotation file<br >
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genome=/n/groups/hbctraining/intro_rnaseq_hpc/reference_data_ensembl38/ensembl38_STAR_index
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- transcriptome=/n/groups/hbctraining/rna-seq_2019_02/reference_data/ salmon_index
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+ transcriptome=/n/groups/hbctraining/rna-seq_2023_02/ salmon_index/ref-transcripts
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gtf=/n/groups/hbctraining/intro_rnaseq_hpc/reference_data_ensembl38/Homo_sapiens.GRCh38.92.1.gtf<br >
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# ; make all of the output directories
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# ; The -p option means mkdir will create the whole path if it
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