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37 | 37 | #' \item The first subset contains barcodes that are selected by the \dQuote{simple filtering algorithm}, which are regarded as high quality cells without any further filtering.
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38 | 38 | #' The minimum threshold \eqn{T} for this subset is defined by taking the \code{max.percentile} percentile of the top \code{n.expected.cells} barcodes,
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39 | 39 | #' and then dividing by the \code{max.min.ratio} to obtain a minimum UMI count.
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40 |
| -#' All barcodes here will have an FDR of zero. |
| 40 | +#' (This is closely related to the algorithm used by \code{\link{defaultDrops}}.) |
| 41 | +#' All barcodes identified in this manner will have an FDR of zero. |
41 | 42 | #' \item The second subset contains the ambient pool and is defined as all barcodes with rankings between \code{ind.min} and \code{ind.max}.
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42 | 43 | #' The barcodes that fall in this category will be used to compute the ambient profile.
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43 | 44 | #' None of these barcodes are considered to be potential cells.
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51 | 52 | #'
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52 | 53 | #' The main differences between \code{emptyDropsCellRanger} and \code{emptyDrops} are:
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53 | 54 | #' \itemize{
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54 |
| -#' \item \code{emptyDropsCellRanger} applies a simple filtering strategy to identify some real cells before any further investigation. |
| 55 | +#' \item \code{emptyDropsCellRanger} does not use the knee point to identify \dQuote{presumed real} cells, |
| 56 | +#' instead relying on a threshold based on the expected number of cells. |
55 | 57 | #' \item \code{emptyDropsCellRanger} takes barcodes whose total count ranks within a certain range - by default, \eqn{(45,000, 90,000]} - to compute the ambient profile.
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56 | 58 | #' In contrast, \code{emptyDrops} only defines the upper bound using \code{lower} or \code{by.rank}.
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57 |
| -#' \item \code{emptyDropsCellRanger} defines a cell candidate pool according to three parameters, \code{umi.min},\code{umi.min.frac.median} and \code{cand.max.n}. |
| 59 | +#' \item \code{emptyDropsCellRanger} defines a cell candidate pool according to three parameters, \code{umi.min}, \code{umi.min.frac.median} and \code{cand.max.n}. |
58 | 60 | #' In \code{emptyDrops}, this is only defined by \code{lower}.
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59 | 61 | #' }
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60 | 62 | #'
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