diff --git a/protos/protos.d.ts b/protos/protos.d.ts
index 4ca4f6b53..0a48bf135 100644
--- a/protos/protos.d.ts
+++ b/protos/protos.d.ts
@@ -1,4 +1,4 @@
-// Copyright 2024 Google LLC
+// Copyright 2025 Google LLC
 //
 // Licensed under the Apache License, Version 2.0 (the "License");
 // you may not use this file except in compliance with the License.
diff --git a/protos/protos.js b/protos/protos.js
index a6b2b37db..88799e49a 100644
--- a/protos/protos.js
+++ b/protos/protos.js
@@ -1,4 +1,4 @@
-// Copyright 2024 Google LLC
+// Copyright 2025 Google LLC
 //
 // Licensed under the Apache License, Version 2.0 (the "License");
 // you may not use this file except in compliance with the License.
diff --git a/src/index.ts b/src/index.ts
index 20cad99c4..ef2969be2 100644
--- a/src/index.ts
+++ b/src/index.ts
@@ -39,7 +39,7 @@ import {
 import * as is from 'is';
 import {Transform, pipeline} from 'stream';
 
-import {entity, Entities, Entity, EntityProto, ValueProto} from './entity';
+import {entity, Entities, Entity, ValueProto} from './entity';
 import {AggregateField} from './aggregate';
 import Key = entity.Key;
 export {Entity, Key, AggregateField};
@@ -125,6 +125,27 @@ const gapic = Object.freeze({
 
 const urlSafeKey = new entity.URLSafeKey();
 
+/**
+ * Retrieves the domain to be used for the service path.
+ *
+ * This function retrieves the domain from DatastoreOptions passed in or via an
+ * environment variable.
+ * @param {string} [prefix] The prefix for the domain.
+ * @param {string} [suffix] The suffix for the domain.
+ * @param {DatastoreOptions} [opts] The gax client options
+ * @returns {string} The universe domain.
+ */
+function getDomain(prefix: string, suffix: string, opts?: DatastoreOptions) {
+  // From https://github.com/googleapis/nodejs-datastore/blob/5c0ddbca91c41e056443eb0b60449f3cdddd6e69/src/v1/datastore_client.ts#L127-L138
+  // This code for universe domain was taken from the Gapic Layer.
+  // It is reused here to build the service path.
+  const universeDomainEnvVar =
+    typeof process === 'object' && typeof process.env === 'object'
+      ? process.env['GOOGLE_CLOUD_UNIVERSE_DOMAIN']
+      : undefined;
+  return `${prefix}.${opts?.universeDomain ?? universeDomainEnvVar ?? suffix}`;
+}
+
 /**
  * Idiomatic class for interacting with Cloud Datastore. Uses the lower-level
  * {@link DatastoreClient} class under the hood.
@@ -489,7 +510,9 @@ class Datastore extends DatastoreRequest {
 
     options.projectId = options.projectId || process.env.DATASTORE_PROJECT_ID;
 
-    this.defaultBaseUrl_ = 'datastore.googleapis.com';
+    const prefixDefault = 'datastore';
+    const suffixDefault = 'googleapis.com';
+    this.defaultBaseUrl_ = `${prefixDefault}.${suffixDefault}`;
     this.determineBaseUrl_(options.apiEndpoint);
 
     const scopes: string[] = Array.from(
@@ -504,7 +527,9 @@ class Datastore extends DatastoreRequest {
         libName: 'gccl',
         libVersion: require('../../package.json').version,
         scopes,
-        servicePath: this.baseUrl_,
+        servicePath: this.customEndpoint_
+          ? this.baseUrl_
+          : getDomain(prefixDefault, suffixDefault, options),
         port: typeof this.port_ === 'number' ? this.port_ : 443,
       },
       options
diff --git a/test/gapic-mocks/get-initialized-datastore-client.ts b/test/gapic-mocks/get-initialized-datastore-client.ts
index bf3e4b652..2a5ded061 100644
--- a/test/gapic-mocks/get-initialized-datastore-client.ts
+++ b/test/gapic-mocks/get-initialized-datastore-client.ts
@@ -12,7 +12,7 @@
 // See the License for the specific language governing permissions and
 // limitations under the License.
 
-import {Datastore} from '../../src';
+import {Datastore, DatastoreOptions} from '../../src';
 
 /**
  * This function gets a datastore client that has already been initialized
@@ -22,15 +22,18 @@ import {Datastore} from '../../src';
  * evaluate data that reaches the handwritten layer thereby testing the
  * handwritten layer in isolation.
  */
-export function getInitializedDatastoreClient(): Datastore {
+export function getInitializedDatastoreClient(
+  opts?: DatastoreOptions
+): Datastore {
   const clientName = 'DatastoreClient';
+  const adminClientName = 'DatastoreAdminClient';
   const PROJECT_ID = 'project-id';
   const NAMESPACE = 'namespace';
   const options = {
     projectId: PROJECT_ID,
     namespace: NAMESPACE,
   };
-  const datastore = new Datastore(options);
+  const datastore = new Datastore(opts ? opts : options);
   // By default, datastore.clients_ is an empty map.
   // To mock out commit we need the map to contain the Gapic data client.
   // Normally a call to the data client through the datastore object would initialize it.
@@ -39,6 +42,13 @@ export function getInitializedDatastoreClient(): Datastore {
   const gapic = Object.freeze({
     v1: require('../../src/v1'),
   });
-  datastore.clients_.set(clientName, new gapic.v1[clientName](options));
+  datastore.clients_.set(
+    clientName,
+    new gapic.v1[clientName](datastore.options)
+  );
+  datastore.clients_.set(
+    adminClientName,
+    new gapic.v1[adminClientName](datastore.options)
+  );
   return datastore;
 }
diff --git a/test/request.ts b/test/request.ts
index 532966420..36a89f336 100644
--- a/test/request.ts
+++ b/test/request.ts
@@ -1886,7 +1886,8 @@ describe('Request', () => {
   });
 
   describe('requestStream_', () => {
-    let GAX_STREAM: gax.CancellableStream;
+    let GAX_STREAM: gax.CancellableStream =
+      new PassThrough() as unknown as gax.CancellableStream;
     const CONFIG = {};
 
     beforeEach(() => {
diff --git a/test/service-path.ts b/test/service-path.ts
new file mode 100644
index 000000000..d8af908fc
--- /dev/null
+++ b/test/service-path.ts
@@ -0,0 +1,102 @@
+// Copyright 2025 Google LLC
+//
+// Licensed under the Apache License, Version 2.0 (the "License");
+// you may not use this file except in compliance with the License.
+// You may obtain a copy of the License at
+//
+//     https://www.apache.org/licenses/LICENSE-2.0
+//
+// Unless required by applicable law or agreed to in writing, software
+// distributed under the License is distributed on an "AS IS" BASIS,
+// WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+// See the License for the specific language governing permissions and
+// limitations under the License.
+
+import {describe, it} from 'mocha';
+import * as assert from 'assert';
+import {DatastoreClient, DatastoreAdminClient} from '../src/v1';
+import {getInitializedDatastoreClient} from './gapic-mocks/get-initialized-datastore-client';
+
+describe('Service Path', () => {
+  it('Setting universe domain should set the service path', async () => {
+    // Set the environment variable
+    process.env.GOOGLE_CLOUD_UNIVERSE_DOMAIN = 'otherDomain';
+
+    const universeDomain = 'someUniverseDomain'; // or your universe domain if not using emulator
+    const options = {
+      universeDomain,
+    };
+    const datastore = getInitializedDatastoreClient(options);
+    assert.strictEqual(
+      (
+        datastore.clients_.get(
+          'DatastoreAdminClient'
+        ) as unknown as DatastoreAdminClient
+      )['_opts'].servicePath,
+      `datastore.${universeDomain}`
+    );
+    assert.strictEqual(
+      (datastore.clients_.get('DatastoreClient') as unknown as DatastoreClient)[
+        '_opts'
+      ].servicePath,
+      `datastore.${universeDomain}`
+    );
+
+    // Clean up the environment variable after the test
+    delete process.env.GOOGLE_CLOUD_UNIVERSE_DOMAIN;
+  });
+  it('Setting universe domain and custom endpoint should set the service path to custom endpoint', async () => {
+    // Set the environment variable
+    process.env.GOOGLE_CLOUD_UNIVERSE_DOMAIN = 'otherDomain';
+
+    const universeDomain = 'someUniverseDomain'; // or your universe domain if not using emulator
+    const apiEndpoint = 'someApiEndpoint';
+    const options = {
+      universeDomain,
+      apiEndpoint,
+    };
+    const datastore = getInitializedDatastoreClient(options);
+    assert.strictEqual(
+      (
+        datastore.clients_.get(
+          'DatastoreAdminClient'
+        ) as unknown as DatastoreAdminClient
+      )['_opts'].servicePath,
+      'someApiEndpoint'
+    );
+    assert.strictEqual(
+      (datastore.clients_.get('DatastoreClient') as unknown as DatastoreClient)[
+        '_opts'
+      ].servicePath,
+      'someApiEndpoint'
+    );
+
+    // Clean up the environment variable after the test
+    delete process.env.GOOGLE_CLOUD_UNIVERSE_DOMAIN;
+  });
+  it('Setting GOOGLE_CLOUD_UNIVERSE_DOMAIN should set the service path', async () => {
+    const universeDomain = 'someUniverseDomain'; // or your universe domain if not using emulator
+
+    // Set the environment variable
+    process.env.GOOGLE_CLOUD_UNIVERSE_DOMAIN = universeDomain;
+    const datastore = getInitializedDatastoreClient(); // No options needed, it will pick up the env var
+
+    assert.strictEqual(
+      (
+        datastore.clients_.get(
+          'DatastoreAdminClient'
+        ) as unknown as DatastoreAdminClient
+      )['_opts'].servicePath,
+      `datastore.${universeDomain}`
+    );
+    assert.strictEqual(
+      (datastore.clients_.get('DatastoreClient') as unknown as DatastoreClient)[
+        '_opts'
+      ].servicePath,
+      `datastore.${universeDomain}`
+    );
+
+    // Clean up the environment variable after the test
+    delete process.env.GOOGLE_CLOUD_UNIVERSE_DOMAIN;
+  });
+});