diff --git a/src/extension/noConfigDebugInit.ts b/src/extension/noConfigDebugInit.ts
index f9787d76..33df8c20 100644
--- a/src/extension/noConfigDebugInit.ts
+++ b/src/extension/noConfigDebugInit.ts
@@ -65,6 +65,8 @@ export async function registerNoConfigDebug(
             fs.unlinkSync(tempFilePath);
         }
     }
+    // clear the env var collection to remove any existing env vars
+    collection.clear();
 
     // Add env var for PYDEVD_DISABLE_FILE_VALIDATION to disable extra output in terminal when starting the debug session.
     collection.replace('PYDEVD_DISABLE_FILE_VALIDATION', '1');