diff --git a/doc/source/whatsnew/v2.2.0.rst b/doc/source/whatsnew/v2.2.0.rst
index 795d277268e44..d36f990240c5b 100644
--- a/doc/source/whatsnew/v2.2.0.rst
+++ b/doc/source/whatsnew/v2.2.0.rst
@@ -358,6 +358,7 @@ Strings
 Interval
 ^^^^^^^^
 - Bug in :class:`Interval` ``__repr__`` not displaying UTC offsets for :class:`Timestamp` bounds. Additionally the hour, minute and second components will now be shown. (:issue:`55015`)
+- Bug in :meth:`IntervalIndex.from_arrays` when passed ``datetime64`` or ``timedelta64`` arrays with mismatched resolutions constructing an invalid ``IntervalArray`` object (:issue:`55714`)
 - Bug in :meth:`IntervalIndex.get_indexer` with datetime or timedelta intervals incorrectly matching on integer targets (:issue:`47772`)
 - Bug in :meth:`IntervalIndex.get_indexer` with timezone-aware datetime intervals incorrectly matching on a sequence of timezone-naive targets (:issue:`47772`)
 - Bug in setting values on a :class:`Series` with an :class:`IntervalIndex` using a slice incorrectly raising (:issue:`54722`)
diff --git a/pandas/core/arrays/interval.py b/pandas/core/arrays/interval.py
index 4bc30d6dbd029..28ee6b2476b0d 100644
--- a/pandas/core/arrays/interval.py
+++ b/pandas/core/arrays/interval.py
@@ -357,6 +357,11 @@ def _ensure_simple_new_inputs(
                 f"'{left.tz}' and '{right.tz}'"
             )
             raise ValueError(msg)
+        elif needs_i8_conversion(left.dtype) and left.unit != right.unit:
+            # e.g. m8[s] vs m8[ms], try to cast to a common dtype GH#55714
+            left_arr, right_arr = left._data._ensure_matching_resos(right._data)
+            left = ensure_index(left_arr)
+            right = ensure_index(right_arr)
 
         # For dt64/td64 we want DatetimeArray/TimedeltaArray instead of ndarray
         left = ensure_wrapped_if_datetimelike(left)
diff --git a/pandas/tests/indexes/interval/test_constructors.py b/pandas/tests/indexes/interval/test_constructors.py
index 9524288b33eef..1efe5ff980f6c 100644
--- a/pandas/tests/indexes/interval/test_constructors.py
+++ b/pandas/tests/indexes/interval/test_constructors.py
@@ -256,6 +256,29 @@ def test_mixed_float_int(self, left_subtype, right_subtype):
         tm.assert_index_equal(result.right, expected_right)
         assert result.dtype.subtype == expected_subtype
 
+    @pytest.mark.parametrize("interval_cls", [IntervalArray, IntervalIndex])
+    def test_from_arrays_mismatched_datetimelike_resos(self, interval_cls):
+        # GH#55714
+        left = date_range("2016-01-01", periods=3, unit="s")
+        right = date_range("2017-01-01", periods=3, unit="ms")
+        result = interval_cls.from_arrays(left, right)
+        expected = interval_cls.from_arrays(left.as_unit("ms"), right)
+        tm.assert_equal(result, expected)
+
+        # td64
+        left2 = left - left[0]
+        right2 = right - left[0]
+        result2 = interval_cls.from_arrays(left2, right2)
+        expected2 = interval_cls.from_arrays(left2.as_unit("ms"), right2)
+        tm.assert_equal(result2, expected2)
+
+        # dt64tz
+        left3 = left.tz_localize("UTC")
+        right3 = right.tz_localize("UTC")
+        result3 = interval_cls.from_arrays(left3, right3)
+        expected3 = interval_cls.from_arrays(left3.as_unit("ms"), right3)
+        tm.assert_equal(result3, expected3)
+
 
 class TestFromBreaks(ConstructorTests):
     """Tests specific to IntervalIndex.from_breaks"""