diff --git a/stdlib/os/__init__.pyi b/stdlib/os/__init__.pyi
index 7289ac425f40..c68a587671a2 100644
--- a/stdlib/os/__init__.pyi
+++ b/stdlib/os/__init__.pyi
@@ -331,8 +331,6 @@ class stat_result:
 @runtime_checkable
 class PathLike(Protocol[_AnyStr_co]):
     def __fspath__(self) -> _AnyStr_co: ...
-    if sys.version_info >= (3, 9):
-        def __class_getitem__(cls, item: Any) -> GenericAlias: ...
 
 @overload
 def listdir(path: str | None = ...) -> list[str]: ...
diff --git a/tests/stubtest_allowlists/py310.txt b/tests/stubtest_allowlists/py310.txt
index 03b972214117..abcec51eb097 100644
--- a/tests/stubtest_allowlists/py310.txt
+++ b/tests/stubtest_allowlists/py310.txt
@@ -51,6 +51,7 @@ ipaddress.IPv6Interface.hostmask
 ipaddress._BaseNetwork.broadcast_address
 ipaddress._BaseNetwork.hostmask
 multiprocessing.spawn._main
+os.PathLike.__class_getitem__  # PathLike is a protocol; we don't expect all PathLike classes to implement class_getitem
 pickle.Pickler.reducer_override  # implemented in C pickler
 # platform.uname_result's processor field is now dynamically made to exist
 platform.uname_result.__new__
diff --git a/tests/stubtest_allowlists/py39.txt b/tests/stubtest_allowlists/py39.txt
index 35480dc7ea65..630414b832d4 100644
--- a/tests/stubtest_allowlists/py39.txt
+++ b/tests/stubtest_allowlists/py39.txt
@@ -46,6 +46,7 @@ ipaddress.IPv6Interface.hostmask
 ipaddress._BaseNetwork.broadcast_address
 ipaddress._BaseNetwork.hostmask
 multiprocessing.spawn._main
+os.PathLike.__class_getitem__  # PathLike is a protocol; we don't expect all PathLike classes to implement class_getitem
 pickle.Pickler.reducer_override  # implemented in C pickler
 # platform.uname_result's processor field is now dynamically made to exist
 platform.uname_result.__new__