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Description
User story
As a developer (Ben) I would like the new BGE pipeline to have a custom labware creator for the final blending step. This labware creator will take multiple pairs of pools: BGE Lib Pool (PCR Free MX pool) and a BGE Cap Lib Pool Norm (ISC MX pool) and create a BGE Blended cap lib pool.
This is a large story (and research) because of the unknowns and because it hasn't been done before in Limber. We will need to deal with tag clashes (maybe using tag_depth) because both pools will have the same exact tags (since they are from the same plate originally). There should be no active requests here but we might want to do an automated submission here for the final part of the pipeline, alternatively users can request additional sequencing for this final post-blending section.
Note if this is not possible we can change the end of the BGE pipeline to just be a normal ISC branch but blending is preferable if possible.
Who are the primary contacts for this story
Matt M, Sara W, Liz C, Katy T, Ben T
Who is the nominated tester for UAT
Matt M, Sara W, Liz C
Acceptance criteria
To be considered successful the solution must allow:
- Using the finalised pipeline diagram for reference (linked below), add and test a labware creator for the blending step.
- Pairs of pools to blend must be from the same initial plate (plate at which pipeline splits) and have matching tags. Tags may be missing from one side of the pool but that doesn't prevent blending.
- There may be more than one pair of tubes depending on how many samples are being pooled together.
Dependencies
This story is blocked by the following dependencies:
- Depends on Limber BGE pipeline story.
Additional context
Research, question and answers, and lucid chart are available in this doc.