IFCB Flow Metric

IFCB Flow Metric is an anomaly detection toolkit for Imaging FlowCytobot (IFCB) data. It extracts statistical features from the ROI (region of interest) point clouds in each IFCB bin and trains an Isolation Forest to identify distributions that deviate from normal patterns. Scores can be visualized through a web dashboard for interactive exploration.

Features

• Parallel feature extraction from IFCB ADC files
• Isolation Forest training for unsupervised anomaly detection
• CSV based scoring of new data sets
• Dash powered dashboard to explore anomaly scores and individual point clouds
• Dockerfile for deployment with Gunicorn

Installation

1. Clone this repository

   git clone https://github.com/WHOIGit/ifcb-flow-metric.git
   cd ifcb-flow-metric

2. Install Python dependencies (Python >=3.11 recommended)

   pip install -r requirements.txt

Training a Model

Use train.py to train an Isolation Forest on a directory of IFCB bins.

python train.py <data_dir> [options]

Options:

• --id-file – path to a file with one PID per line. If omitted, all bins in data_dir are used.
• --n-jobs – number of parallel workers for feature extraction (default from utils/constants.py).
• --contamination – expected fraction of anomalies.
• --aspect-ratio – camera frame aspect ratio.
• --chunk-size – number of PIDs per extraction chunk.
• --model – output path for the trained model (default classifier.pkl).
• --config – YAML string specifying which features to use for training.
• --config-file – YAML file path specifying which features to use for training.

A typical command might look like:

python train.py /path/to/data --n-jobs 4 --contamination 0.00001

Feature Selection

By default, all 26 available features are used for training. You can customize which features to include using either:

1. YAML configuration file:

   python train.py /path/to/data --config-file feature_config.yaml

2. YAML string directly:

   python train.py /path/to/data --config 'spatial_stats: {mean_x: true, mean_y: true}'

The repository includes feature_config.yaml as an example configuration file with all features enabled. Features are organized into categories:

• Spatial Statistics (8 features): mean, std, median, IQR for x/y coordinates
• Distribution Shape (2 features): ratio_spread, core_fraction
• Clipping Detection (2 features): duplicate_fraction, max_duplicate_fraction
• Histogram Uniformity (2 features): cv_x, cv_y
• Statistical Moments (4 features): skew_x, skew_y, kurt_x, kurt_y
• PCA Orientation (2 features): angle, eigen_ratio
• Edge Features (5 features): left/right/top/bottom/total edge fractions
• Temporal (1 feature): t_y_var

The trained model is stored as a pickle file for later inference.

Scoring Data

To compute anomaly scores for a set of bins using a trained model:

python score.py <data_dir> [options]

Important options:

• --id-file – list of PIDs to score.
• --n-jobs – workers for feature extraction.
• --aspect-ratio – camera aspect ratio.
• --chunk-size – PIDs per extraction chunk.
• --model – path to the saved model.
• --output – CSV file to write results (default scores.csv).

Each row in the CSV contains a PID and its anomaly score.

Running the Dashboard

dashboard.py provides a Dash application for exploring scores. It reads the CSV produced by score.py and fetches point cloud data from the IFCB dashboard API.

python dashboard.py

The dashboard URL defaults to http://localhost:8000 but can be changed via the DASHBOARD_BASE_URL environment variable. Additional environment variables include FILE_PATH (path to the scores CSV), MONTH (filter data by month in YYYYMM format), and DECIMATE (plotting decimation factor).

Docker

The repository includes a Dockerfile for running the dashboard under Gunicorn:

docker build -t ifcb-flow-metric .
docker run -p 8050:8050 -v /path/to/scores.csv:/app/scores.csv ifcb-flow-metric

This exposes the dashboard on port 8050.

Repository Overview

Path	Description
models/	Feature extraction, training, and inference utilities
utils/	Helper functions and constants
train.py	Command line training script
score.py	Command line scoring script
dashboard.py	Dash dashboard for interactive exploration

Default configuration values such as contamination rate and output paths are defined in utils/constants.py.

License

This project is licensed under the MIT License. See the LICENSE file for details.

Authors

Some of this code and most of this README were generated by AI.