ChatSpatial replaces ad-hoc LLM code generation with schema-enforced orchestration. Instead of generating arbitrary scripts, the LLM selects tools and parameters from a curated registry, making spatial transcriptomics workflows more reproducible across sessions and clients.
It exposes 60+ spatial transcriptomics methods as MCP tools, so any MCP-compatible client can analyze data through natural language.
- Install ChatSpatial — Installation Guide
- Configure your MCP client — Configuration Guide
- Run your first analysis — Quick Start
Minimal example prompt:
Load /absolute/path/to/spatial_data.h5ad and show me the tissue structure
ChatSpatial works with any MCP-compatible client — Claude Code, Claude Desktop, Codex, OpenCode, and other MCP-capable tools.
60+ methods across 11 categories. Supports 10x Visium, Xenium, Slide-seq v2, MERFISH, seqFISH.
| Category | Methods |
|---|---|
| Spatial Domains | SpaGCN, STAGATE, GraphST, BANKSY, Leiden, Louvain |
| Deconvolution | FlashDeconv, Cell2location, RCTD, DestVI, Stereoscope, SPOTlight, Tangram, CARD |
| Cell Communication | LIANA+, CellPhoneDB, CellChat (cellchat_r), FastCCC |
| Cell Type Annotation | Tangram, scANVI, CellAssign, mLLMCelltype, scType, SingleR |
| Differential Expression | Wilcoxon, t-test, Logistic Regression, pyDESeq2 |
| Trajectory & Velocity | CellRank, Palantir, DPT, scVelo, VeloVI |
| Spatial Statistics | Moran's I, Local Moran, Geary's C, Getis-Ord Gi*, Ripley's K, Co-occurrence, Neighborhood Enrichment, Centrality Scores, Local Join Count, Network Properties |
| Enrichment | GSEA, ORA, Enrichr, ssGSEA, Spatial EnrichMap |
| Spatial Genes | SpatialDE, SPARK-X, FlashS |
| Integration | Harmony, BBKNN, Scanorama, scVI |
| Other | CNV Analysis (InferCNVPy, Numbat), Spatial Registration (PASTE, STalign) |
| Guide | Owns |
|---|---|
| Installation | Environment setup, package install, platform notes |
| Quick Start | First successful analysis after setup |
| Concepts | Method selection and analysis reasoning |
| Examples | Prompt recipes and workflow examples |
| Configuration | Exact MCP client configuration syntax |
| Troubleshooting | Symptom → fix guidance |
| Methods Reference | Canonical tool parameters and defaults |
| Full Docs | Complete documentation site |
If you use ChatSpatial in your research, please cite:
@article{Yang2026.02.26.708361,
author = {Yang, Chen and Zhang, Xianyang and Chen, Jun},
title = {ChatSpatial: Schema-Enforced Agentic Orchestration for Reproducible and Cross-Platform Spatial Transcriptomics},
elocation-id = {2026.02.26.708361},
year = {2026},
doi = {10.64898/2026.02.26.708361},
publisher = {Cold Spring Harbor Laboratory},
URL = {https://www.biorxiv.org/content/early/2026/03/01/2026.02.26.708361},
journal = {bioRxiv}
}ChatSpatial orchestrates many excellent third-party methods. Please also cite the original tools your analysis used.
Documentation improvements, bug reports, and new analysis methods are all welcome. See CONTRIBUTING.md.